Lab Manual in Biochemistry, Immunology and Biotechnology by Arti Nigam, , available at Book Depository with free delivery. Get this from a library! Lab manual in biochemistry, immunology and biotechnology. [Arti Nigam; Archana Ayyagari]. Lab Manual in Biochemistry, Immunology and Biotechnology. Front Cover. Arti Nigam, Archana Ayyagari. Tata McGraw-Hill Pub., – Biochemistry –

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The E-mail Address es you entered is are not in a valid format. Your rating has been recorded. To monitor catalase activity in the given aerobic tissue sample. My library Help Advanced Book Search.

Lab Manual in Biochemistry, Immunology and Biotechnology

To estimate the levels of cellulases in the given sample. To purify IgG from serum sample by using the biotechnolgoy of affinity chromatography and confirm the result by double immunodiffusion.

The specific requirements or preferences of your reviewing publisher, classroom teacher, institution or organization should be applied. To estimate acid phosphatase activity and to obtain the progress curve of the enzyme.

To carry out ammonium sulfate precipitation of amylase enzyme present in the crude protein extract. To separate biotechnologu mixture of sugars glucose and maltose by thin layer chromatography.

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To carry out nigsm transduction of E.

Lab Manual in Biochemistry, Immunology and Biotechnology by Archana Ayyagari,Arti Nigam –

She did her post graduation in biotechnology from Jawaharlal Nehru University inand was subsequently awarded her Ph D in Microbiology in from the University of Delhi South Campus. Latest techniques of immunology 3. To prepare a master plate and carry out replica plating of the master plate. Immunology and Biotechnology Nigam No preview available – To carry out screening test for glucosephosphate dehydrogenase, and its estimation in the given blood sample.


To carry out isolation, purification and culture of protoplast. To detect syphilis spirochaete using indirect fluorescent antibody IFA technique. To demonstrate the presence of cholesterol and its esters microscopically by Schultz’s method.

To determine the blood group of given blood sample by slide agglutination. To determine total leucocyte count TLC of the given blood sample. To perform single radial immunodiffusion by Mancini’s technique. To purify the plasmid DNA obtained by: To test urine sample for bile salts and bile pigments.

To estimate invertase using commercially available yeast tablets as the enzyme source. To carry out serological examination for diagnosis of syphilis. Other editions – View all Lab Manual in Biochemistry: To estimate ascorbic acid by titration using 2,6 – dichlorophenol indophenol.

Lab manual in biochemistry, immunology and biotechnology

Written by authors who have over a decade of experience in teaching and research, this book will serve the needs of: To study typical prokaryotic and eukaryotic cells i microscope.

Read, highlight, and take notes, across web, tablet, and phone. To carry out qualitative tests for the presence of proteins in the given sample. To study complement fixation and to calculate the antibody titre in the given serum sample.


To determine the saponification value of a fat. To perform homogenization of rat liver tissue, and obtain its subcellular fractions.

To isolate chromosomal DNA from E. Please verify that you are not a robot. Citations are based on reference standards. To perform Barfoed’s test for distinguishing reducing monosaccharides from reducing disaccharides. Check out the top books of the year on our page Best Books of English View all editions and formats Summary:.

Estimation of amylase enzyme activity I. To detect the presence of amino acids qualitatively by Ninhydrin test, Lqb test, Glyoxylic test, modified Millon’s test, Pauly’s test, Ehrlich test, Nitroprusside test, lead acetate test and Sakaguchi test.

To carry out routine analysis of the given urine sample for i physical appearance ii chemical tests. Some features of WorldCat will not be available.

Lab manual in biochemistry, immunology and biotechnology (Book, ) []

To link the enzyme horse radish peroxidase with anti-BSA. To estimate protein quantitatively by Biuret method. Preview this item Preview this item. Search WorldCat Find items in libraries near you. Find a copy in the library Finding biochemistrry that hold this item Your list has reached the maximum number of items. Looking for beautiful books?

To perform Seliwanoff’s test for detection of ketose sugars. To purify messenger RNA by affinity chromatography technique.