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Gluconeogenesis in Leishmania mexicana. Gluconeogenesis is an active pathway in Leishmania amastigotes and is essential for their survival within the mammalian cells. However, our knowledge about this pathway in trypanosomatids is very limited.
GK participates in the entry of glycerol in promastigotes and amastigotes; PEPCK participates in the entry of aspartate in promastigotes, and PPDK is involved in the entry of alanine in amastigotes. Furthermore, the majority of alanine enters into the pathway via decarboxylation of pyruvate in promastigotes, whereas pathway redundancy is suggested for the entry of aspartate in amastigotes.
Interestingly, we also found that l-lactate, an abundant glucogenic precursor in mammals, was used by Leishmania amastigotes to synthesize mannogen, entering the pathway through PPDK. On the basis of these new results, we propose a revision in the current model of gluconeogenesis in Leishmania, emphasizing the differences between amastigotes and promastigotes. This work underlines the importance of studying the trypanosomatid intracellular life cycle stages to gain a better understanding of the pathologies caused in humans.
What is Sambucus mexicana Adoxaceae? Inconsistent application of the name Sambucus mexicana Presl ex DC. It matches plants from the area around Monterey, Califo La Artesania Mexicana Mexican Handicrafts.
This booklet contains instructions in English and Spanish for making eleven typical Mexican craft articles. The instructions are accompanied by pen-and-ink drawings. Seguridad Laboral al Usar Pesticidas. Spasmogenic and spasmolytic activities of Agastache mexicana ssp.
Agastache mexicana has been used in traditional medicine for relief of abdominal pain and treatment of other diseases.
Christina Tio Trisnasari’s – – Page 6
Two subspecies have been identified: To determine the effect of methanol extracts of A. A comparative chromatographic profile of these extracts was also done to indicate the most abundant flavonoids presents in methanol extracts of both subspecies.
This spasmogenic effect was partially inhibited by atropine, prolematica of muscarinic receptors; and pyrilamine, antagonist of H 1 receptors. The spasmolytic activity of AMX was partially inhibited by hexamethonium, ganglionic blocker; and indomethacin, inhibitor of the synthesis of prostaglandins; but not by L-NAME, inhibitor of nitric oxide synthase.
Chromatographic analyses of these methanol extracts showed the presence of acacetin and tilanin in both.
These results suggest that in folk medicine only AMX should be used as spasmolytic, and not in combination with AMM as traditionally occurs, due to the spasmogenic effects of the latter.
In addition, activation of nicotinic receptors, prostaglandins and calcium channels, but. Isolation of Leishmania mexicana from Neotoma Micropus.
Other vertebrates such as opossums, hispid cotton rats, and armadillos and other sand flies such as Lutzomyia diabolica and Lutzomyia texana Leishmania mexicana by a North American sand fly, Lutzomyia anthophora Diptera: Journal of Medical Entomology Contributions of Zea mays subspecies mexicana haplotypes to modern maize. Maize was domesticated from lowland teosinte Zea mays ssp.
Here, two genomes for Mo17 a modern maize inbred and mexicana are assembled using a meta-assembly strategy after sequencing of 10 lines derived from a maize-teosinte cross. Comparative analyses reveal a high level of diversity between Mo17, B73, and mexicanaincluding three Mb-size structural rearrangements.
The maize spontaneous mutation rate is estimated to be 2. A higher deleterious mutation rate is observed in the pericentromeric regions, and might be caused by differences in recombination frequency. Our data offer a rich resource for constructing the pan-genome of Zea mays and genetic improvement of modern maize varieties.
Photoacoustic monitoring of life cycles of Leishmania Mexicana. Photoacoustic spectroscopy is used to monitor in situ, the difference between the two forms of the protozoan Leishmania Mexicana.
Differences are the result of changes in the respiratory chain and could be attributed, according to our results, to the presence of cytochrome b in promastigotes and cytochrome c in amastigotes. Leishmania Leishmania mexicana causes cutaneous leishmaniasis, an endemic zoonosis affecting a growing number of patients in the southeastern states of Mexico.
Some foci are found in shade-grown cocoa and coffee plantations, or near perennial forests that provide rich breeding grounds for the sand fly vectors, but also harbor a variety of bat species that live off the abundant fruits provided by these shade-giving trees.
The close proximity between sand flies and bats makes their interaction feasible, yet bats infected with Leishmania L. Here we analyzed bats from six states of Mexico that had reported patients with leishmaniasis.
We found that 41 bats 9.
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The infected tissues showed no evidence of macroscopic lesions. Of the infected bats, 12 species were frugivorous, insectivorous or nectarivorous, and only one species was sanguivorous Desmodus rotundusand most of them belonged to the family Phyllostomidae. The eco-region where most of the infected bats were caught is the Gulf Coastal Pproblematica of Chiapas and Tabasco.
Through experimental infections of two Tadarida brasiliensis bats in captivity, we show that this species can harbor viable, infective Leishmania L. We conclude that various species of bats belonging to the family Phyllostomidae are possible reservoir hosts for Leishmania L. Further studies are needed to determine how these bats become infected, how long the parasite remains viable inside these potential hosts and whether they are infective to sand flies to fully evaluate their impact on disease epidemiology.
Hacen falta estudios longitudinales sobre el papel de la diabetes como factor de riesgo para demencia. Objetivo Determinar el riesgo de demencia en sujetos Mexicanos con diabetes mellitus tipo 2. El riesgo de desarrollar demencia en estos individuos fue el doble RR, 2. Seguridad del paciente en Radioterapia Intraoperatoria: Impacto de los elementos controlados por el Radiofisico.
En la administracion de la radioterapia intervienen profesionales y equipos de tratamiento, por lo que existe el riesgo de error y se precisa que dicho equipamiento funcione conforme a lo esperado. A los radiofisicos les corresponde participar en las actividades de garantia o aseguramiento de la calidad, incluyendo el control de calidad de los equipos, y en la evaluacion de los riesgos asociados.
La radioterapia intraoperatoria RIO es una tecnica radioterapica de intensificacion de dosis, altamente selectiva, dirigida a volumenes anatomicos restringidos durante el tratamiento quirurgico oncologico, basada en la administracion de una dosis absorbida alta por medio de un haz de electrones tras el examen visual directo del lecho tumoral. Como incorporar los ultimos avances en el refuerzo de la seguridad en radioterapia es una tarea ambiciosa y compleja, resulta mas concreta y de inmediata aplicacion su introduccion en la RIO.
El objetivo es analizar los elementos que reducen los riesgos y aumentan la seguridad en la RIO y su dosimetria, y valorar la funcion del radiofisico en esta labor. Se realizo un analisis de modos de fallo y efectos failure mode and effect analysis, FMEA con el fin de identificar los elementos que forman la RIO y aplicar las herramientas necesarias para la minimizacion de los riesgos y la mejora de la seguridad en la tecnica.
Se estudiaron las verificaciones diarias de dicho acelerador Precise con el control estadistico de procesos statistical process control, SPC y se simularon intervenciones para devolverlo al estado llamado en control. The institutions involved in this scope have the compromise to interchange and share the data and advice to the Civil Protection authorities.
Background Leishmaniasis is a worldwide disease prevalent in tropical and sub tropical countries. Many attempts have been made and different strategies have been approached to develop a potent vaccine against Leishmania.
DNA immunisation is a method, which is shown to be effective in Leishmania vaccination. However, the CTL activity does not necessarily correlate with the protection induced by the vaccine. Also, gene gun immunisation is a potential approach in Leishmania vaccination.
These findings would be helpful in opening new windows in Leishmania vaccine research. In vitro activity of Tridax procumbens against promastigotes of Leishmania mexicana. Tridax procumbens is an active herb against leishmaniasis.
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Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicanathe causative agent of cutaneous leishmaniasis in the New World. Extracts and 3S ,didehydrofalcarinol 1 were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated.
In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT. We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. In this study we report the biological activity of extracts and 3S ,didehydrofalcarinol 1obtained from Tridax procumbens, on the promastigote form of Leishmania mexicanawith no effect upon mammalian cells. La region de Los Angeles contiene una red de fallas activas, incluyendo muchas fallas por empuje que son profundas y no rompen la superficie de la tierra.
Estas fallas ocultas incluyen la falla anteriormente desconocida que fue responsable por la devastacion que ocurrio durante el terremoto de Northridge en enero deel terremoto mas costoso en la historia de los Estados Unidos.
El Experimento Sismico en la Region de Los Angeles Los Angeles Region Seismic Experiment, LARSEesta localizando los peligros ocultos de los terremotos debajo de la region de Los Angeles para mejorar la construccion de las estructuras que pueden apoyar terremotos que son inevitables en el futuro, y que ayudaran a los cientificos determinar donde occurira el sacudimento mas fuerte y poderoso.
Recurrence of Mexican long-tongued bats Choeronycteris mexicana at historical sites in Arizona and New Mexico. The Mexican long-tongued bat Choeronycteris mexicana is a nectar-eating species that seasonally inhabits the southwestern United States. Sincefewer than individuals of C. We conducted a field survey in Arizona and New Mexico during summer to check historically occupied areas for recurrence of C.
Christina Tio Trisnasari’s
Multiple individuals were observed at many sites, including young-of-year. Choeronycteris mexicana roosted in lighted areas close to entrances within mine adits, abandoned buildings, wide rock crevices, and caves. All occupied sites were in Madrean evergreen woodlands or semidesert grasslands where species of Agave were present. Most sites were located near a water source and, with the exception of a single site, near areas of riparian vegetation. Sites at which we did not encounter C.
Based on the relatively high rate of bat recurrence, we do not believe that populations of C. The california poppy eschscholtzia mexicana as a copper indicator plant – a new example. The abundance and distribution of the California poppy Eschscholtzia mexicana correlates closely with the copper-rich outcrop of a small porphyry-type deposit in Arizona.
Chemical factors are probably more histogia than physical factors in determining why this species is sometimes found as a copper indicator plant. Mechanism of action of relaxant effect of Agastache mexicana ssp. This study investigates the relaxant action mechanism of A. Essential oil composition was estragole, d-limonene and linalyl anthranilate. Also, AMEO antagonized the calcium chloride-induced contractions.
The results suggest that relaxant effect of AMEO might be due to blockade of calcium influx in guinea-pig trachea smooth muscle.