年1月23日 CalcuSyn is a software tool whose main purpose is to aid individuals in analyzing mixed drug treatments, a more and more used practice in. combination-index data generated by CalcuSyn software analyses .. and antagonism, respectively (Source: CalcuSyn manual, Biosoft, ). Manual and Software, Biosoft, Cambridge, U.K., Chou, T.-C. and Hayball , M. CalcuSyn for Windows, Multiple-drug dose-effect analyzer and manual.
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Using the Program 27 Entering a Combination Drug As with entering a single drug there are two methods to enter a combination drug: It is determined from the x-intercept of the median-effect plot.
Analysis of drug interactions.
Graphs The export graph mwnual opens when you select File Export from within a graph window. Using the Program 41 imports text file data directly into the raw data grid where you can then use it to create drugs and combinations within your CalcuSyn experiment. Synergism at what dose level?
Instead, potentiation augmentation, enhancement or inhibition suppression can be determined. CalcuSyn was reviewed by Madalina Boboc. For combinations, in contrast, any number of data points can be used for the Manusl calculations.
Welcome to COMPUSYN
Multiple drug effect analysis with confidence interval. State that the drugs are in constant ratio to each other and enter the ratio of the two drugs to each other e. Three data files are installed with CalcuSyn in its Samples folder. Double-click on Experiment Details to enter the experiment properties. However, if too many effect levels are selected, the isobolograms frequently become messy and difficult to read.
Data can be entered via the keyboard or file import either into the grid or directly into the analysis engine. Mixed drug treatment is becoming common in the treatment of cancer, AIDS etc, and CalcuSyn is the most widely used software for establishing efficacy in this field. Manual and Software, Biosoft, Cambridge, U. Some of the early applications have been described in the review articles given in Ref. Extensive Help contents are provided and thus, it becomes obvious that both power and novice users can find their way around it, without facing any kind of difficulties.
These ambiguous practices generate confusion. This is the second drug Dm Note that in the two drug combination, the CI is the sum of three terms with the third term being the product of the first two terms. Click OK to complete the entry. For two drug combinations, we may define from Eq.
This will open the raw data import dialog that lets you import either files or ODBC data. MTX Fraction affected uM 0.
Analysis of drug interactions.
Analysis of combined drug effects: Quantitative dose-effect analysis and algorithms: Calcksyn 7 – Click OK and the drug will be added to the experiment and shown in the contents pane. Cisplatin Data points entered: Note that the CD version has Autorun and, if your computer is suitable, that will present a menu on-screen from which you can select the Install option.
Any text or numbers appearing before this are ignored. The errors of estimate can be from several sources, e. By contrast, the classic non-normalized isobologram not only shows the degrees of synergism or antagonism at specified effect levels but also at specified dose concentration levels. You can alter these drugs at a later time using the Select Summary Drugs Using the Program 29 The combinations will then be added to the experiment and shown in the Contents Pane. The DRI is derived from a ratio of doses as below: A measure of how much the dose of each drug in a synergistic combination may be reduced at a given effect level compared with the doses of each drug alone The goodness of fit for the data to the manula equation is represented by the linear correlation coefficient r of the median-effect plot.
In a simple system such as enzyme or receptor, it is analogous to noncompetitive inhibition between two inhibitors. As indicated by the multiple drug-effect equation of Chou-Talalay 6, 8each term of the equation is the dividing ratio and, therefore, maanual unit for each term single drug term or drug combination term cancels out and becomes a dimensionless quantity.
Several relevant points need to be considered: Computerized quantitation of synergism and antagonism of taxol, topotecan and cisplatin against teratocarcinoma cell growth: This will open the first page of the wizard.
This is the first drug Data points entered: An equation determines only the additive effect rather than synergism or antagonism. The summary table gives a one-line summary of the drug parameters for each component and for the combination drug. Once you have entered the correct password, you will be able to run CalcuSyn repeatedly.
Your order reference code or license number. The median-effect principle and the combination index for quantitation of synergism and antagonism. Combination of more than Two Drugs For three drug combinations e. This terminology was formerly introduced by J.
In conclusion, CalcuSyn is an efficient piece of software which can help people in creating new mixed drug treatment and more effective ones. Accuracy of Measurement and Conformity to the Mass-action Law The linear correlation coefficient, r, of the median-effect plot should be reasonably good.